Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana
نویسندگان
چکیده
The potential use of algae in biofuels applications is receiving significant attention. However, none of the current algal model species are competitive production strains. Here we present a draft genome sequence and a genetic transformation method for the marine microalga Nannochloropsis gaditana CCMP526. We show that N. gaditana has highly favourable lipid yields, and is a promising production organism. The genome assembly includes nuclear (~29 Mb) and organellar genomes, and contains 9,052 gene models. We define the genes required for glycerolipid biogenesis and detail the differential regulation of genes during nitrogen-limited lipid biosynthesis. Phylogenomic analysis identifies genetic attributes of this organism, including unique stramenopile photosynthesis genes and gene expansions that may explain the distinguishing photoautotrophic phenotypes observed. The availability of a genome sequence and transformation methods will facilitate investigations into N. gaditana lipid biosynthesis and permit genetic engineering strategies to further improve this naturally productive alga.
منابع مشابه
Corrigendum: Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana
متن کامل
Genomic insights from the oleaginous model alga Nannochloropsis gaditana
Nannochloropsis species have emerged as leading phototrophic microorganisms for the production of biofuels. Several isolates produce large quantities of triacylglycerols, grow rapidly, and can be cultivated at industrial scales. Recently, the mitochondrial, plastid and nuclear genomes of Nannochloropsis gaditana were sequenced. Genomic interrogation revealed several key features that likely fac...
متن کاملComplete Genome Sequence of the Model Oleaginous Alga Nannochloropsis gaditana CCMP1894
The model oleaginous alga Nannochloropsis gaditana was completely sequenced using a combination of optical mapping and next-generation sequencing technologies to generate one of the most complete eukaryotic genomes published to date. The assembled genome is 30.7 Mb long.
متن کاملDraft Genome Sequence of Cryptococcus terricola JCM 24523, an Oleaginous Yeast Capable of Expressing Exogenous DNA
Cryptococcus terricola JCM 24523 has recently been identified as an oleaginous yeast capable of converting starch into fatty acids. This draft genome sequence provides a platform for elucidating its fatty acid production potential and supporting comparisons with other oleaginous species.
متن کاملDraft Genome Sequence of the Oleaginous Green Alga Tetradesmus obliquus UTEX 393
The microalgae Tetradesmus obliquus is able to maintain a high photosynthetic efficiency under nitrogen limitation and is considered a promising green microalgae for sustainable production of diverse compounds, including biofuels. Here, we report the first draft whole-genome shotgun sequencing of T. obliquus The final assembly comprises 108,715,903 bp with over 1,368 scaffolds.
متن کامل